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Marrrta [24]
2 years ago
5

During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM Tris (

pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how much of each stock do you need to make 500ml of the A150 buffer?
Biology
1 answer:
vesna_86 [32]2 years ago
7 0

Answer:

You will need 10 mL of stock Tris, 15mL of stock NaCl and enough water to bring the total volume to 500 mL.

Explanation:

<u>The desired buffer contains:</u>

  • Total volume of 500 mL
  • Tris 30mM
  • NaCl 150mM

<u>The stock solutions are:</u>

  • 1.5M = 1500mM Tris
  • 5M = 5000mM NaCl

We can use the formula C1V1=C2V2 to calculate the volume of each stock required to prepare the buffer.

<h2><u>Tris</u></h2>
  • C1= 1500 mM
  • V1=?
  • C2= 30mM
  • V2= 500 mL

1500 mM x V1 = 30mM x 500 mL

V1 = (30mM x 500 mL) / 1500 mM

V1 = 10 mL

<h2><u>NaCl</u></h2>
  • C1= 5000 mM
  • V1=?
  • C2= 150 mM
  • v2= 500 mL

5000 mM x V1 = 150mM x 500 mL

V1 = (150 mM x 500 mL) / 5000 mM

V1 = 15 mL

You will need 10 mL of stock Tris, 15mL of stock NaCl and enough water to bring the total volume to 500 mL.

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<h2>Learn more about:  </h2>
  • Glycogen: brainly.com/question/6359375
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