Calculate the approximate percent increase in luciferase activity between cells containing plasmid pCD3γ-59 and cells containing
plasmid pCD3γ-149 . Round to the nearest whole number.
1 answer:
Answer:
Explanation:
CD3γ comprises of various polypeptide gamma chain genes which are promoters for regulatory sequences that might have positive or negative effects on expression of the gene.
A luciferase assay acitivity is a reporter assay that is commonly used to make enquiry about expression of genes at the transcription. It is used to make enquiry if a protein can activate or repress the expression of a target gene.
The data file attached below; shows the record of the Analysis of the luciferase activity of T lymphocytes containing plasmids with different amounts of the CD3γ regulatory sequence.
We will see that the pCD3γ-59 contains the core promoter sequence as it possess the maximum effect on Luciferase expression. To calculate the increase in luciferase activity ; we will need more data to perform more deletions for a confirmatory result in order to calculate the increase in luciferase activity
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