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Ludmilka [50]
2 years ago
5

You are asked to study a new inhibitor of oxidative phosphorylation. When it is added to a suspension of mitochondria carrying o

ut this process, both ATP synthesis and oxygen consumption are inhibited. You suspect that the inhibitor blocks either the F-type ATPase or the electron transport chain. You can perform an experiment that measures the consumption of oxygen and the synthesis of ATP by isolated mitochondria. In one paragraph (6 sentences maximum), explain how the uncoupler, dinitrophenol, can be used to determine whether the inhibitor blocks the ATPase or the electron transport chain.
This was all the information that was given.
Biology
1 answer:
scoundrel [369]2 years ago
8 0

Answer:

DNP produces the loss of the proton gradient (i.e., the energy of the proton gradient is dissipated in the form of heat instead to produce ATP)

Explanation:

2,4-Dinitrophenol (DNP) acts to shuttle H+ ions across cellular membranes, bypassing the ATP synthase used by mitochondria to generate ATP during cellular respiration. Since DNP is able to bypass ATP synthase, this compound uncouples the phosphorylation of ADP by the ATP synthase from the process of oxidation (i.e., transport of electrons). For example, in muscle cells, DNP may be used to shuttle calcium ions (Ca +) from mitochondrial stores, and free intracellular Ca+ ions are evidenced to produce muscle contraction.

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In direct calorimetry, a person is placed in a large, water-insulated chamber. The chamber is kept at a constant temperature. Wh
Anastaziya [24]

Answer:

Although direct calorimetry is considered to be the most precise technique for the measurement of metabolic rate, construction of such a chamber that is large enough for doing exercise physiology research (or any research for that matter) is extremely expensive and not at all mobile.

Explanation:

Direct calorimetry measures the heat production of an individual, in calories, when placed in an insulated chamber where the heat is transferred to surrounding water. This is a very accurate method of measuring energy expenditure.

5 0
2 years ago
Organisms that eat cows do not obtain a great deal of energy from the cows. In fact, they get less energy than the cows obtain f
marshall27 [118]

Answer:

The cow uses all but stores ten percent of what they eat.

Explanation:

For example, a plant produces 100 percent energy. The plant needs to use the energy for survival.

When a cow eats the plant, it only gains 10 percent of the energy.

Then, say a wolf eats the cow.

The wolf only gets 1 percent of the energy that is produced by the plant.

5 0
1 year ago
Imagine that you are asked to measure the rate of respiration for a 25 g reptile and a 25 g mammal at 10 degrees Celsius. Predic
Airida [17]
The body temperature of the mammal will be higher than that of the reptile. this is because the mammal body temperature does not depend on its environmental temperature while a reptile does not have a stable body temperature and depend on the temperature of its environment.
8 0
2 years ago
g Indicate true (T) and false (F) statements below regarding cytokinesis in animal cells. Choose the correct answer represented
frozen [14]
<h2>Cytokinesis in animal cells</h2>

Explanation:

  • The force for cytokinesis is generated by kinesin motors on microtubule bundles that form the contractile ring:This statement is false because cytokinesis in animal cells starts with the assembly of contractile ring,contractile band consists of actin and myosin(microfilaments) which catalyse cleavage furrow formation
  • As the contractile ring constricts, its thickness increases to keep a constant volume:This statement is false because thickness remains constant
  • The midbody forms from bundles of actin and myosin: This statement is false because contractile ring forms bundles of actin and myosin whereas midbody is formed from microtubules
  • Local activation of Ran GTPase triggers the assembly and contraction of the contractile ring:This statement is false because local activation of Rho mediates profilin binding to actin and helps in assembly and contraction of contractile ring

The four letter string comes out to be FFFF

6 0
2 years ago
In writing On the Origin of Species, Darwin synthesized ideas from other publications, as well as observations he made while voy
algol [13]

Answer:

Principles of Geology

Explanation:

J. Henslow gifted Darwin the first volume of Principles of Geology, written by Charles Lyell. The book explains about the old age of the earth. Accordingly, the slow rate of several geological processes suggests that the earth is very old.

Darwin read the book as the ship was crossing the Atlantic and learned about geological processes occurring over the millions of years. For example, he learned that the earth's surface was not formed by some catastrophic event.

In his book, Lyell explained the slow and gradual geological processes like erosion gave rise to the vast landscapes present on the earth's surface.

Darwin compared the time taken by geological processes to form the earth's surface and reasoned if these millions of years were enough to support the evolution of species.

6 0
1 year ago
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