Answer:
D.-
Explanation:
A control group is needed to carry out the experiment, that control group is the one that is not injected with anything. Ethanol and pesticide need to be injected into separated groups to document whether it is one or the other that is harmful
The correct answer is <u /><em>a</em><em>daption</em>. The particular environment in which a particular environment is exposed to gives it the characteristic or the ability to adapt. This is how a particular ecosystem works. It is the relationship of organisms within their environment that evolves through time in order for it to survive and propagate into more adapted kinds of species.
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Answer:
a) The response indicates that a pH below or above this range will most likely cause enolase to denature/change its shape and be less efficient or unable to catalyze the reaction.
b)The response indicates that the appropriate negative control is to measure the reaction rate (at the varying substrate concentrations) without any enzyme present.
c)The response indicated that the enolase has a more stable/functional/correct/normal protein structure at the higher temperature of 55°C than at 37°C because the enzyme is from an organism that is adapted to growth at 55°C.
Explanation:
Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis.In bacteria, enolases are highly conserved enzymes and commonly exist as homodimers.
The temperature optimum for enolase catalysis was 80°C, close to the measured thermal stability of the protein which was determined to be 75°C, while the pH optimum for enzyme activity was 6.5. The specific activities of purified enolase determined at 25 and 80°C were 147 and 300 U mg−1 of protein, respectively. Km values for the 2-phosphoglycerate/phosphoenolpyruvate reaction determined at 25 and 80°C were 0.16 and 0.03 mM, respectively. The Km values for Mg2+ binding at these temperatures were 2.5 and 1.9 mM, respectively.
Enolase-1 from Chloroflexus aurantiacus (EnoCa), a thermophilic green non-sulfur bacterium that grows photosynthetically under anaerobic conditions. The biochemical and structural properties of enolase from C. aurantiacus are consistent with this being thermally adapted.
Answer:
PFK: cytoplasm
insulin: ER--> Golgi--> outside cell
Explanation:
As described in the question, the enzyme phosphofructokinase (PFK) functions is a cells cytoplasm. Enzymes are proteins and proteins are made in the ribosomes of a cell. As ribosomes are present in the cytoplasm, hence PFK once made in the ribosomes will be released into the cytoplasm.
As for insulin, it will be made in the ribosomes. from there, it will move to the Endoplasmic Reticulum for packaging. Then to the Golgi complex for modifications and further packaging. Only after these stages, it will be ready to move out from where it is produces to areas where it has to be used.
Answer:
34
Explanation:
Electron transport begins with serval molecules of NADH and FADH2
