Tennis ball, flowers, and some other stuff.
Answer is: coupled transport differs from primary active transport due to the fact that it uses electrochemical potential difference as a direct source of energy.
Primary active transport directly uses metabolic energy (adenosine triphosphate- ATP) to transport molecules across a membrane.
In secondary active transport (coupled transport) there is no direct coupling of ATP, <span>energy derived from the pumping of protons across a cell membrane.</span>
<span>For the first step, you must isolate the cells from the media. The cells contain the DNA so you keep the cells and pellet.
The next step would be a cell lysis which causes the cells to open and the DNA to come out. At this point you would keep the supernatant, as the pellet is the cell membrane and other parts of the cell.
The chelex is to bind to transition metal ions. This would cause and DNases in the cell to become inactive because the metal ion is their cofactor. The pellet in this case would contain the chelex and the DNA would still be in the supernatant.</span>
