The R group should have the ability to form hydrogen bonds.
The R-group take a lot of different chemical forms and can
be acidic, basic, polar or non polar. Amino acids that are either acidic or basic
consequently have either positive or negative charge. These types of R-groups
are stabilized when surrounded by water.
Answer:
a) The response indicates that a pH below or above this range will most likely cause enolase to denature/change its shape and be less efficient or unable to catalyze the reaction.
b)The response indicates that the appropriate negative control is to measure the reaction rate (at the varying substrate concentrations) without any enzyme present.
c)The response indicated that the enolase has a more stable/functional/correct/normal protein structure at the higher temperature of 55°C than at 37°C because the enzyme is from an organism that is adapted to growth at 55°C.
Explanation:
Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis.In bacteria, enolases are highly conserved enzymes and commonly exist as homodimers.
The temperature optimum for enolase catalysis was 80°C, close to the measured thermal stability of the protein which was determined to be 75°C, while the pH optimum for enzyme activity was 6.5. The specific activities of purified enolase determined at 25 and 80°C were 147 and 300 U mg−1 of protein, respectively. Km values for the 2-phosphoglycerate/phosphoenolpyruvate reaction determined at 25 and 80°C were 0.16 and 0.03 mM, respectively. The Km values for Mg2+ binding at these temperatures were 2.5 and 1.9 mM, respectively.
Enolase-1 from Chloroflexus aurantiacus (EnoCa), a thermophilic green non-sulfur bacterium that grows photosynthetically under anaerobic conditions. The biochemical and structural properties of enolase from C. aurantiacus are consistent with this being thermally adapted.
The hotspots are regions, where the plumes of magma are present just below the lithosphere. The plume of the magma is the particles of the volcano and the gases, which is erupted during the volcanic eruption. It is generated by the fragmentation of the magma. Once, it reaches the lithosphere, it get spreaded laterally.
The plumes at the hotspots are present just below the tectonic plates, a high temperature r heat and the low pressure causes the rocks present in the lithosphere to melt resulting in volcanic eruption. At hotspot, the melting of rock takes time, sometimes it is very slow, due to the presence of various tectonic plates. Hence, the plumes can remain stationary for a very long period of time without erupting.
So, the first blank can be filled with Lithosphere and the second blank can be filled with Stationary.
The new hypotheses proposed by different scientists are often criticized by the others because these new hypotheses contradict the ideas and hypotheses that already exist. Adapting one's mind to a new hypotheses explaining a past event is often difficult when more clear hypotheses were already given earlier. Thus, the hypotheses suggesting the extinction of the dinosaurs are difficult for the scientific community to accept.
Answer:
B. In the absence of oxygen, cells release ATP by breaking down
ethanol into glucose and water.
Explanation: