Answer:
A 22 to 25 amino acid sequence present in the central section of the protein, which gives rise to an alpha helix in the membrane is known as the stop-transfer anchor sequence. The sequence plays an essential function in targeting the protein towards the plasma membrane. On the other hand, it also ceases targeting of the protein towards the endoplasmic reticulum, which was started by the signal peptide.
Thus, the process of translation of the remaining of the protein occurs within the cytosol due to the tethering of the transmembrane domain. In the stop-transfer anchor sequence, the hydrophobic amino acids present are isoleucine and valine. After mutation, these amino acids get converted into arginine and lysine, thus, hydrophilic amino acids replace hydrophobic amino acids in the sequence.
Due to this, the transmembrane domain cannot be targeted towards an integral part of the plasma membrane by the short transfer anchor sequence, and therefore, now the translocation of the protein will take place towards the endoplasmic reticulum as initiated by the signal peptide at the beginning.
