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cestrela7 [59]
2 years ago
12

A researcher is interested in performing indirect immunofluorescence microscopy in human tissue using an antibody raised in goat

s that can detect a human protein called PCNT. Which of the following antibodies would better work as the secondary antibody in this experiment? Goat anti-goat linked to Cy3 Rat anti-goat linked to Cy3 Human anti-human linked to Cy3 Goat anti-human linked to Cy3
Biology
1 answer:
UNO [17]2 years ago
7 0

Answer:

Rat anti-goat linked to Cy3

Explanation:

Indirect immunofluorescence microscopy is a technique used to localize endogenous marker proteins, having this approach high sensitivity. Secondary antibodies are produced by immunization of the target species with antibodies of one 'second' species. In a standard immunofluorescence assay, the secondary antibodies are linked to fluorophores and incubated with the primary antibody. In this case, the Cy3 fluorescent probe is used for labeling secondary antibodies, it is a fluorophore that absorbs light at 550 nm and emits at 570 nm.

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Water, dietary fiber, bacterial cells, and worn-out intestinal cells are components of Gastrointestinal track. This track consists of mouth, esophagus, stomach, small intestine, large intestine, rectum, and anus.This track helps the body for digestion and absorption of nutrients. This is also known as gut, or alimentary canal.


3 0
2 years ago
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Suppose concordance data were collected from a cohort study of twins to study the genetic contribution to the onset of type 1 di
Dmitry_Shevchenko [17]

Answer:

The heritability of type 1 diabetes is likely high, indicating that genetics plays a larger role in the development of type 1 diabetes than the environment.

Explanation:

Identical twins are formed when a single zygote separates into two embryos after the process of fertilization. Hence, these twins arise from the same egg and sperm.

Fraternal twins arise when two different sperms fertilize two eggs at the same time.

Hence, we can say that the genotype of the identical twins is similar.

The results from concordance data prove that genetics play a higher role in type 1 diabetes.

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2 years ago
One human impact on the phosphorus cycle occurs through:
valentina_108 [34]
D is the awnser i think

6 0
2 years ago
Two students are doing an experiment. They mix 40 grams of baking soda with 23 grams of vinegar inside a closed container and wa
natka813 [3]
63 grams is the correct answer

4 0
2 years ago
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When voltage-gated sodium ion channels are inactivated, the neuron is _________________. in the absolute refractory period below
Ymorist [56]

Answer:

depolarizing

Explanation:

According to my research on studies by different medical professionals, I can say that based on the information provided within the question the neuron is depolarizing. This is what happens when voltage-gated sodium ion channels are inactivated causing an increas in the neuron's membrane conductance for sodium ions.

I hope this answered your question. If you have any more questions feel free to ask away at Brainly.

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