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cestrela7 [59]
2 years ago
12

A researcher is interested in performing indirect immunofluorescence microscopy in human tissue using an antibody raised in goat

s that can detect a human protein called PCNT. Which of the following antibodies would better work as the secondary antibody in this experiment? Goat anti-goat linked to Cy3 Rat anti-goat linked to Cy3 Human anti-human linked to Cy3 Goat anti-human linked to Cy3
Biology
1 answer:
UNO [17]2 years ago
7 0

Answer:

Rat anti-goat linked to Cy3

Explanation:

Indirect immunofluorescence microscopy is a technique used to localize endogenous marker proteins, having this approach high sensitivity. Secondary antibodies are produced by immunization of the target species with antibodies of one 'second' species. In a standard immunofluorescence assay, the secondary antibodies are linked to fluorophores and incubated with the primary antibody. In this case, the Cy3 fluorescent probe is used for labeling secondary antibodies, it is a fluorophore that absorbs light at 550 nm and emits at 570 nm.

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Also, remember, the fly trap is a carnivorous plant, while the lotus is not
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The specific heat of a metal is 0.5 cal/ (0C.g). The heat required to raise temperature of 100 gram of that metal by 20 0C is
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The phosphorus in all biological tissues can be traced back to ________.
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Answer:

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Where are carbohydrates found in the body? And in cells?
slavikrds [6]

Hi,

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3 0
2 years ago
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Which one of the following activities is not an example of incident coordination? A. Establishing priorities among incidents. B.
andrew-mc [135]

Answer:

Option B is correct because through out all the activities, controlling, directing and controlling in not a part of incident coordination activities.

Explanation:

To understand this question, it is necessary to understand that who is incident coordinator?  He’s a person who is responsible for tasks related to administration process and ensure that onsite activities are working properly or not. For further explanation, following are the activities that are solely responsibility of incident coordinator:

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• To collect and analyse the send information

• To establish the incident priorities

• To track the resources

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These are the main functions or activities done by the incident coordinator. He is the managing person and cannot control the authorities or give any sort of directions to any person in the department or office. Same as, he is not responsible or direct to give any orders to the staff members.  

Answer Detail:

Level: High School

Subject: Biology

Keywords:

• Incident coordinator

• Shareholder

• Stakeholder

Learn more to evaluate:

Who is shareholder? brainly.com/question/12175451

Administrative work?  brainly.com/question/11671451

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