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cestrela7 [59]
2 years ago
12

A researcher is interested in performing indirect immunofluorescence microscopy in human tissue using an antibody raised in goat

s that can detect a human protein called PCNT. Which of the following antibodies would better work as the secondary antibody in this experiment? Goat anti-goat linked to Cy3 Rat anti-goat linked to Cy3 Human anti-human linked to Cy3 Goat anti-human linked to Cy3
Biology
1 answer:
UNO [17]2 years ago
7 0

Answer:

Rat anti-goat linked to Cy3

Explanation:

Indirect immunofluorescence microscopy is a technique used to localize endogenous marker proteins, having this approach high sensitivity. Secondary antibodies are produced by immunization of the target species with antibodies of one 'second' species. In a standard immunofluorescence assay, the secondary antibodies are linked to fluorophores and incubated with the primary antibody. In this case, the Cy3 fluorescent probe is used for labeling secondary antibodies, it is a fluorophore that absorbs light at 550 nm and emits at 570 nm.

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A terrarium is a small, enclosed ecosystem. If you change certain aspects of the terrarium, the climate that exists inside it wi
Free_Kalibri [48]

Answer: Add a different diet and maybe add an animal. If you put a bigger water bowl than the cage will become humid. If you change things about the terrain, its a desert so watering more plants and planting trees will make the ground more fertile to other plants. If you continue to take care of the plants until they are able to grow and reproduce. After enough time, where used to be a desert there will be a forest.

6 0
1 year ago
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A line graph has a vertical axis labeled "Average plant mass (g)^ * and a horizontal axis labeled "Herbicide (g/plant)." What in
SSSSS [86.1K]

Answer:

The correct option is D. The average mass of plants exposed to 3 g of herbicide was 10g.

Explanation:

As the vertical axis is labelled as average plant mass, it will make the y- coordinate.

As the horizontal axis is labelled as Herbicide (g/plant), it will make the x-axis.

The coordinates for the given value are (3,10). 3 will be the value of the x- coordinate. Hence, it will show the average mass of the plant at the plotted point which will be 3 g.

10 will show the value of the y- coordinate which will be the amount of herbicide given to the plant.

Hence, Dis the correct option.

7 0
2 years ago
What component of enzyme structure is responsible for enzyme specificity
son4ous [18]
A few enzymes<span> exhibit absolute </span>specificity<span>; that is, they will catalyze only one particular reaction. Other </span>enzymes<span> will be specific for a particular type of chemical bond or functional group. In general, there are four distinct types of </span>specificity<span>: Absolute </span>specificity<span> - the </span>enzyme<span> will catalyze only one reaction.</span>
4 0
2 years ago
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In three to five sentences, describe the advantages and disadvantages of these maps in modeling mitosis.
zhenek [66]

Answer:

Mitosis involves in cell division.

Explanation:

Mitosis may be defined as a process of cell division which results in the formation of two daughter cells each from a parent cell and each cell having having the same number of chromosomes and chromosomes of the same type as that of the parent nucleus.

The main difference between meiosis and mitosis of a nucleus cell is that it involves only one cell division. And so the cell goes by the steps of prophase, then metaphase, then anaphase and finally telophase. The specialized cells could not survive independently to each other, so they have to develop some complex system of communications like the circulatory system and the nervous system. In this way they can survive. If one of the system dies, entire organisms is more likely to follow.

3 0
2 years ago
The transport of molecules of a particular solute from inside an animal cell across the cell membrane to the extracellular fluid
Leona [35]

Answer:

the concentration of the solute is lower inside the cell than outside it

Explanation:

This question depicts the process of ACTIVE TRANSPORT, which is the movement of a substance against concentration gradient, hence, requires energy input (ATP) to occur. In this case, transporting a solute from inside an animal cell to the extracellular fluid across the cell membrane always requires energy.

This is because the concentration of solute inside the cell is much lower than that of the extracellular fluid, hence, to move the solutes against this concentration gradient (low to high), energy in form of ATP is required.

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2 years ago
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