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cestrela7 [59]
2 years ago
12

A researcher is interested in performing indirect immunofluorescence microscopy in human tissue using an antibody raised in goat

s that can detect a human protein called PCNT. Which of the following antibodies would better work as the secondary antibody in this experiment? Goat anti-goat linked to Cy3 Rat anti-goat linked to Cy3 Human anti-human linked to Cy3 Goat anti-human linked to Cy3
Biology
1 answer:
UNO [17]2 years ago
7 0

Answer:

Rat anti-goat linked to Cy3

Explanation:

Indirect immunofluorescence microscopy is a technique used to localize endogenous marker proteins, having this approach high sensitivity. Secondary antibodies are produced by immunization of the target species with antibodies of one 'second' species. In a standard immunofluorescence assay, the secondary antibodies are linked to fluorophores and incubated with the primary antibody. In this case, the Cy3 fluorescent probe is used for labeling secondary antibodies, it is a fluorophore that absorbs light at 550 nm and emits at 570 nm.

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An example of a highly vascular tissue is ________ tissue. an example of a tissue that is not highly vascular is ________.
inn [45]
The answer to the first unknown in the problem is the "Areolar" while in the second unknown in the problem is the "Cartilage". Hence, to complete the given statement in the problem, we have an example of a high vascular tissue is "AREOLAR" tissue and as an example also of a tissue that is not highly vascular is the "CARTILAGE".
5 0
1 year ago
A particular ant species has four pairs of chromosomes. Identify the genetic possibilities from independent assortment of a game
pantera1 [17]
In this question, the ant has 4 pairs of chromosomes. Each pair of chromosomes will be separated in a gamete, resulting 4 unpaired chromosomes. That is why gamete is called haploid or n.
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Then for 4 chromosomes would be 4^4 possibilities= 256 possibilities of offspring
8 0
1 year ago
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ki77a [65]

The correct option is this: THE MASS OF THE PROBE REMAINS THE SAME, BUT THE WEIGHT OF THE PROBE DECREASES.

The mass of an object is a measure of the amount of substance it contains and this amount always remain the same unless there is a change in quantity of matter. This is the reason why the mass of a substance will remain constant, no matter where it is placed, whether on planet earth or on another planet. The weight of a substance on the other hand is a force, whose value depends on the force of gravity. Thus, the force of gravity that exist in the place, where the object is placed will determine its weight.

7 0
1 year ago
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Sholpan [36]
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4 0
2 years ago
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Paul [167]

Answer: C. The environment changed suddenly from a shallow ocean setting to a river system.

Explanation:

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The fact that the oceanic fossils are beneath the river system layer means that the ocean came first and the river system layer came second. The environment must therefore have been a shallow ocean setting that changed into a river system.

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