Answer:
The type of vesicular transport involved in the exporting of protein-based hormones, such as insulin, into the bloodstream is regulated exocytosis.
Explanation:
In general terms, exocytosis is a type of active transport that allows intracellular substances are released to the extracellular space, through of vesicles that fuse with the plasma membrane, which allow the exit of substances from inside the cell.
Regulated exocytosis is the specific vesicular transport for the secretion of substances, such as hormones. For this type of transport to exist, the presence of an extracellular signal is required, which will activate the fusion of the vesicles.
In the case of insulin, the external signal originates with the increase in blood glucose levels, a signal that penetrates the intracellular space and generates an increase in insulin production in the islets of Langerhans (pancreas).
Before insulin secretion occurs, the cell must be depolarized, allowing calcium to enter, which promotes transport by regulated exocytosis of insulin to the extracellular space.
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The color changes from clear to blue, then once heated to orange.
I did this experiment not too long ago so hopefully this helps! (:
<span>For the first step, you must isolate the cells from the media. The cells contain the DNA so you keep the cells and pellet.
The next step would be a cell lysis which causes the cells to open and the DNA to come out. At this point you would keep the supernatant, as the pellet is the cell membrane and other parts of the cell.
The chelex is to bind to transition metal ions. This would cause and DNases in the cell to become inactive because the metal ion is their cofactor. The pellet in this case would contain the chelex and the DNA would still be in the supernatant.</span>
