Explanation:
During PCR, we use two primers one is forward primer and the other one is reverse primer they match the sequence of one one of the two complementary strands of the target DNA, they flank the target region (that the region which we has to be copied). if we add only one primer it copies only one strand of the DNA in multiple copies. Usually we call this as Asymmetric PCR.
Generally Primers are synthetic short stretch of oligonucleotides that are complementary to the target DNA. They act as a foundation for the amplification process of DNA to form multiple copies
Answer:
Hypertonic blood draws water out of the interstitial fluid, which makes the interstitial fluid hypertonic. This, in turn, draws water out of the cells.
Basically an hype tonic blood has high solute potential,( low water potential) compare with the surrounding plasma and the interstitial fluid. Thus the interstitial fluid is hypotonic to the blood.
Consequently,water with higher potential moves from the interstitial fluid medium into the blood by osmosis through the capillary endothelial. This raises the water potential of the blood, lowering the solute potential, thus making it hypotonic to the interstitial fluid; which is now hypertonic(lower water potential ,due to loss to the blood by osmosis).
Since the interstitial fluids is now hyper tonic to the surrounding cells, water moves from the hypo tonic surrounding cells through osmosis into the interstitial fluids. The sequence continues until a stable internal environment is achieved,
Answer:
From the given information, the transport of glucose in blood across the red blood cells takes place by facilitated diffusion, that involves the spontaneous passive-transport with the help of trans-membrane proteins or transporter proteins to move the ions or molecules down the concentration gradient.
While the transport of glucose in intestines take place via active transport mechanism. It takes place from gut into intestines. It occurs against the concentration gradient with the help of energy molecules like proteins.
Answer:
Hydrogen Bonds
Explanation:
In the polymerase chain reaction (PCR) the temperature rises to 90 ° C - 95 ° C, to break the hydrogen bonds, which are the types of bonds responsible for pairing the two strands of DNA, this process is known as denaturation of DNA.
The DNA is extremely stable, due to a large number of bonds (hydrogen bonds) that form between the two strands. If the temperature decreases, these bonds will begin to recompose, until the DNA returns to its original state
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