Question

A scientist who has isolated the green fluorescent protein (gfp) gene from a sample of jellyfish dna would use which technology to make gfp protein from the gfp gene most effectively?

Answer 1

To make GFP protein from the GFP gene most effectively is done by combining in the ratios of  4: 2 to make a bunch of copies of the coding DNA, then he pops that coding sequence into an expression vector.
Afterward, he uses that vector to transform the bacteria to make them usable as protein-making factories. The ratio 2 would only make enough DNA's, whereas the ratio 4 will be used to make the protein.

Answer 2

The most efficient way to make more GFP is the gene cloning by using bacteria which you have to put the GFP gene on it. The more genes (bacteria) you will have, the more efficient the production of GFP will be.

About the other answers in the MCQ:
The gel electrophoresis only separates proteins or DNA
The PCR could help to obtain more genes to incorporate but it is not a necessity
The restriction digestive enzyme will help us to obtain a specific gene that we want. (it's probably what the scientist use to isolate the GFP gene)