Due to the definition of the central dogma, another way of putting it is that the central dogma follows the flow of information from DNA to protein.
Answer:
a) The response indicates that a pH below or above this range will most likely cause enolase to denature/change its shape and be less efficient or unable to catalyze the reaction.
b)The response indicates that the appropriate negative control is to measure the reaction rate (at the varying substrate concentrations) without any enzyme present.
c)The response indicated that the enolase has a more stable/functional/correct/normal protein structure at the higher temperature of 55°C than at 37°C because the enzyme is from an organism that is adapted to growth at 55°C.
Explanation:
Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis.In bacteria, enolases are highly conserved enzymes and commonly exist as homodimers.
The temperature optimum for enolase catalysis was 80°C, close to the measured thermal stability of the protein which was determined to be 75°C, while the pH optimum for enzyme activity was 6.5. The specific activities of purified enolase determined at 25 and 80°C were 147 and 300 U mg−1 of protein, respectively. Km values for the 2-phosphoglycerate/phosphoenolpyruvate reaction determined at 25 and 80°C were 0.16 and 0.03 mM, respectively. The Km values for Mg2+ binding at these temperatures were 2.5 and 1.9 mM, respectively.
Enolase-1 from Chloroflexus aurantiacus (EnoCa), a thermophilic green non-sulfur bacterium that grows photosynthetically under anaerobic conditions. The biochemical and structural properties of enolase from C. aurantiacus are consistent with this being thermally adapted.
Answer:
C
Explanation: A fish can live in water and get carbon in the air bubbles but it's not enough carbon to thrive off of so the fish may need to eat other fish or plankton in the water. The second example A wolf would get its carbon from both the air and water but would still need extra carbons so that is why it would hunt a deer to consume its carbons. Also, the deer would get its carbons from the air, water, and grass. the grass would get its carbons from water and air. NOW DO YOU GET IT EVERYTHING IS CARBONS AND LIVES OFF OF THEM AND IT WILL ALWAYS BE THAT WAY!
I believe i the correct answer is c because he notices that some of the traits weren't being passed on.
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