Answer:
Rat anti-goat linked to Cy3
Explanation:
Indirect immunofluorescence microscopy is a technique used to localize endogenous marker proteins, having this approach high sensitivity. Secondary antibodies are produced by immunization of the target species with antibodies of one 'second' species. In a standard immunofluorescence assay, the secondary antibodies are linked to fluorophores and incubated with the primary antibody. In this case, the Cy3 fluorescent probe is used for labeling secondary antibodies, it is a fluorophore that absorbs light at 550 nm and emits at 570 nm.
It is best to use regular light microscopes to observe that.
For simple structures, onion cells doesn't require a very large magnification. Magnifications for such as 100X or 150X is already enough to determine the different structures of onion cells such as cell walls or cytoplasm.
It is not very suitable to use a electron microscope especially as high school or college students, as electronic microscopes can be really expensive, usually only top universities have them. Also, it is quite complicated to control the microscope comparing to the common light microscope that we can easily find in many school laboratories. They're easy to function and they're a lot cheaper.
So, it's best advised to use a light microscope, unless if you're really into studying very detailed structures and you're experienced in doing these sorts of things, then you should use a electron microscope.
Answer: Annelida
Leeches are soft bodied segmented parasitic worms. They belong to the phylum Annelida. They are hermaphrodites which means they exhibit sex organs of both male and female.
The characteristics which are found in leeches which group them into Annelida are:
1. The body of the leeches are bilaterally symmetrical like other Annelids. The body organs are also segmented.
2. They have a closed circulatory system in which the heart pumps the blood to all the parts of the body.
3. Leeches either exhibit a protusible pharynx or proboscis which allows them to suck the blood of the host such features are also common in other Annelids.
Answer:
When the body has excess water, thirst is suppressed. An interaction between the pituitary gland and the kidneys provides another mechanism. When the body is low in water, the pituitary gland secretes vasopressin (also called antidiuretic hormone) into the bloodstream.
