Answer:
Basically the translation of CK-B protein is inhibited in the U937D cells, despite the fact that the CK-B protein is bounded to the ribosomes. This is because(<u>mechanisms)</u> the translation is inhibited by the binding of translational repressors to the 3’UTR of the CK-B mRNA rather than the actual CK-B mRNA 3'UTR.
Furthermore, the soluble protein inhibitions is due to the reaction of the U937 cells to the short RNA sequences with the 3’UTR.
<u>The introduction of these sequences(shot segement of RNA) </u>into the U937D cells leads to CK-B synthesis. This makes 3’UTR sequences to bind to the translational repressor proteins, thus preventing them from binding to the CK-B mRNA
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COMPLETED QUE.
A common feature of many eukaryotic mRNAS is the presence of a rather long 3' UTR, which often contains consensus sequences. Creatine kinase B (CK-B) is an enzyme important in cellular metabolism. Certain cells—termed U937D cells—have lots of CK-B mRNA, but no CK- B enzyme is present. In these cells the 5’ end of the CK-B mRNA is bound to ribosomes, the mRNA is apparently not translated. Something inhibits the translation of the CK-B mRNA in these cells. Researchers introduced numerous short segments of RNA containing only 3’UTR sequences into U937D cells. As result, the U937D cells began to synthesize the CK-B enzyme, but the total amount of CK-B mRNA did not increase. The introduction of short segments of other RNA sequences did not stimulate the synthesis of CK-B; only the 3’UTR sequences turned on the translation of the enzyme. Based on these results, <u>purpose a mechanism for how CK-B translation is inhibited in U937D cells. Explain how the introduction of short segments of RNA containing the 3'UTR sequences might remove the inhibition.</u>
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Explanation:
Answer: Because the interval between isolines is constant, their spacing gives an visual indication of the change that occurs over a given distance, called a gradient. Hope this helps :)
Explanation:
When Sarah performed this experiment, she changed the levels of nitrogen in order to observe the changes in the morphological features of the plant.
All of the characteristics of the plant that she observed were dependent on multiple different things, but the level of nitrogen was an independent variable that she was changing at her will.
Answer:didn’t put the number for how many blue cups were used
Explanation:
The answer is Dilutions
Bacterial culture is one of the laboratory examination that could be used to determine if a specimen contain bacteria. But it will hard to count the colony if the bacteria count is too high.
That is why the specimen of the culture need to be diluted to reduce the bacteria count of the specimen, so the result will be easier to count. The unit used would be colony forming unit(CFU) per milliliter.
