Answer:
Pneumonia may be defined as the medical condition in which the inflammation might occur in the lungs due to which the fluid might accumulate in the lungs. The infection might occur by the Streptococcus penumoniae bacteria.
The most likely diagnosis of this patient can be influenza. The symptoms shown by the patient like cough, fever, headache, lung pain is more common in influenza. The diagnosis can be based on the symptoms shown by the patient and no presence of the Streptococcal infection. This conditions can be worsened if the pneumonia follow the harsh influenza and this secondary infection must be monitored. The treatment that can be preferred in this patient bed rest, anti pyretic medicines and proper body fluids.
Invasive species cause harm to wildlife in many ways. When a new and aggressive species is introduced into an ecosystem, it may not have any natural predators or controls. ... Invasive species can change the food web in an ecosystem by destroying or replacing native food sources.
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Answer:
a) The response indicates that a pH below or above this range will most likely cause enolase to denature/change its shape and be less efficient or unable to catalyze the reaction.
b)The response indicates that the appropriate negative control is to measure the reaction rate (at the varying substrate concentrations) without any enzyme present.
c)The response indicated that the enolase has a more stable/functional/correct/normal protein structure at the higher temperature of 55°C than at 37°C because the enzyme is from an organism that is adapted to growth at 55°C.
Explanation:
Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis.In bacteria, enolases are highly conserved enzymes and commonly exist as homodimers.
The temperature optimum for enolase catalysis was 80°C, close to the measured thermal stability of the protein which was determined to be 75°C, while the pH optimum for enzyme activity was 6.5. The specific activities of purified enolase determined at 25 and 80°C were 147 and 300 U mg−1 of protein, respectively. Km values for the 2-phosphoglycerate/phosphoenolpyruvate reaction determined at 25 and 80°C were 0.16 and 0.03 mM, respectively. The Km values for Mg2+ binding at these temperatures were 2.5 and 1.9 mM, respectively.
Enolase-1 from Chloroflexus aurantiacus (EnoCa), a thermophilic green non-sulfur bacterium that grows photosynthetically under anaerobic conditions. The biochemical and structural properties of enolase from C. aurantiacus are consistent with this being thermally adapted.
