The mitochondria. An organelle with a very curious history....
Answer:
a) The response indicates that a pH below or above this range will most likely cause enolase to denature/change its shape and be less efficient or unable to catalyze the reaction.
b)The response indicates that the appropriate negative control is to measure the reaction rate (at the varying substrate concentrations) without any enzyme present.
c)The response indicated that the enolase has a more stable/functional/correct/normal protein structure at the higher temperature of 55°C than at 37°C because the enzyme is from an organism that is adapted to growth at 55°C.
Explanation:
Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis.In bacteria, enolases are highly conserved enzymes and commonly exist as homodimers.
The temperature optimum for enolase catalysis was 80°C, close to the measured thermal stability of the protein which was determined to be 75°C, while the pH optimum for enzyme activity was 6.5. The specific activities of purified enolase determined at 25 and 80°C were 147 and 300 U mg−1 of protein, respectively. Km values for the 2-phosphoglycerate/phosphoenolpyruvate reaction determined at 25 and 80°C were 0.16 and 0.03 mM, respectively. The Km values for Mg2+ binding at these temperatures were 2.5 and 1.9 mM, respectively.
Enolase-1 from Chloroflexus aurantiacus (EnoCa), a thermophilic green non-sulfur bacterium that grows photosynthetically under anaerobic conditions. The biochemical and structural properties of enolase from C. aurantiacus are consistent with this being thermally adapted.
Answer:
The endomembrane system includes the nuclear envelope.
Explanation:
The endomembrane is composed by the endoplasmic reticulum (ER) and the golgi apparatus. Its main function is to synthesize proteins (rough endoplasmic reticulum studded with ribosomes) proteins can also be modified in the ER of pass to the golgi apparatus where they can be modified and sort to be packed in vesicles to be exported to different parts of the body, for example hormones.
the nuclear envelope only holds the DNA and it´s not involved in protein synthesis.
Amoebas are single-celled organisms, which means that they are composed of just one cell. Each amoeba is a cell capable of performing all living functions by itself. They can reproduce asexually. They are protozoans with no fixed shape. Most have no hard parts and look like blobs of jelly.
Answer:
The correct answer is B
Explanation:
Transposons need to regulate their copy number to avoid errors with chromosomal pairing during meiosis and mitosis such as unequal crossover.
A typical example of this error is called the Alu Sequence or Elements. Alu elements contain more than one million copies found everywhere in the genome of human beings.
Many inherited human diseases such as cancer are related to Alu insertions.
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