Answer:
a) The response indicates that a pH below or above this range will most likely cause enolase to denature/change its shape and be less efficient or unable to catalyze the reaction.
b)The response indicates that the appropriate negative control is to measure the reaction rate (at the varying substrate concentrations) without any enzyme present.
c)The response indicated that the enolase has a more stable/functional/correct/normal protein structure at the higher temperature of 55°C than at 37°C because the enzyme is from an organism that is adapted to growth at 55°C.
Explanation:
Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis.In bacteria, enolases are highly conserved enzymes and commonly exist as homodimers.
The temperature optimum for enolase catalysis was 80°C, close to the measured thermal stability of the protein which was determined to be 75°C, while the pH optimum for enzyme activity was 6.5. The specific activities of purified enolase determined at 25 and 80°C were 147 and 300 U mg−1 of protein, respectively. Km values for the 2-phosphoglycerate/phosphoenolpyruvate reaction determined at 25 and 80°C were 0.16 and 0.03 mM, respectively. The Km values for Mg2+ binding at these temperatures were 2.5 and 1.9 mM, respectively.
Enolase-1 from Chloroflexus aurantiacus (EnoCa), a thermophilic green non-sulfur bacterium that grows photosynthetically under anaerobic conditions. The biochemical and structural properties of enolase from C. aurantiacus are consistent with this being thermally adapted.
Answer:
The correct answer is -
1. hyaline cartilage
2. fibrocartilage
3. hyaline cartilage
Explanation:
1. The hyaline cartilage covers the articular cartilage of the synovial joint that is translucent or clear as glass-like cartilage that has a firm consistency and a high amount of collagen.
2. A symphysis is an amphiarthrotic joint which is a fibrocartilagenous joint that joins with the adjacent bone. This is a very tough or strong tissue.
3. The growth plate of the long bone or epiphyseal plates are examples of synchondrosis,which is a hyaline cartilage composed tissue.
Answer:
Plant Reproduction and Life Cycle. The life cycle of a plant is very different from the life cycle of an animal. Humans are made entirely of diploid cells (cells with two sets of chromosomes, referred to as ''2n''). ... Plants, however, can live when they are at the stage of having haploid cells or diploid cells.
Explanation:
In an animal life cycle, male and female parents each create sex cells (sperm and eggs) that unite to form a fertilized egg and develop into an offspring organism. Plants, likewise, have sperm and eggs in their life cycles, but these are produced by an intermediate stage between the adult and the offspring.
The correct answer is B. Pond
Oceans and lakes have huge diversity when it comes to temperature, while lagoons also have them except they're not as vast.
Answer:
The use of land that is probably the least important to Carmen and her family is commercial.
Explanation:
The reason behind this is that considering that all the families are farmers. They are most likely to develop an autonomous self-sufficient community in which factories and big businesses have no place. Also, we could consider recreational, however, this type of community is very recreative and use to camp a lot as well as to create parks and public spaces to share and live together.
