If they remain isolated for a long period of time, the two groups will probably diverge genetically, and may result in speciation.
Genetic divergence is the process whereby two groups of the same ancestral species acquire autonomous genetic changes through time, usually after the groups have been separated reproductively for some period of time.
Speciation is an evolutionary process whereby populations develop to become different species.
Plasma proteins account at least 8% of the plasma, they contribute to osmotic pressure and maintain water balance in blood and tissues, other functions includes transport and enzymatic activities. Plasma proteins include albumin, fibrinogen and globulin (alpha, beta and gamma). Globulin proteins may be used to fight infections such that gamma globulin antibodies are released by the plasma during immune response.
The question must refer to the modern classification system in biology.
It started with the work of Linnaeus - so its early version can be called a Linnaean classification - but right now it has been expanded and changed to fit the evolutionary relationships between the species - which is the best description, i.e. the answer is "it is based on the evolutionary relationship of the species"
The independent variable of an experiment is the one that is intentionally changed or manipulated to see the results of changing said variable.
We are told that in each different group, the amount of caffeine that is given to the dogs changes - in the control group, they get no caffeine, in the 2nd group, they get 10 mg of caffeine each, and in the 3rd group, they get 50 mg of caffeine each.
Therefore, since the amount of caffeine that is given to the dogs is the only variable that is being manipulated, this is the independent variable.