Explanation:
During PCR, we use two primers one is forward primer and the other one is reverse primer they match the sequence of one one of the two complementary strands of the target DNA, they flank the target region (that the region which we has to be copied). if we add only one primer it copies only one strand of the DNA in multiple copies. Usually we call this as Asymmetric PCR.
Generally Primers are synthetic short stretch of oligonucleotides that are complementary to the target DNA. They act as a foundation for the amplification process of DNA to form multiple copies
<h3>Question from alternative source</h3>
Many different types of mutations can occur within the body. Cystic fibrosis is a genetic disorder that is caused by different mutations within the CFTR gene. One mutation in the CFTR gene that leads to cystic fibrosis causes translation to end earlier in the gene sequence. Which type of mutation causes this translation error that leads to cystic fibrosis?
- silent
- missense
- nonsense
- deletion
Answer:
Nonsense
Explanation:
Nonsense mutations describe mutations that produce a premature stop codon. The stop codons are UAG, UAA, and UGA. A way in which this could arise is, for example, if there was a substution of a nucleotide in the mRNA codon UAC (which codes for the amino acid Tyrosine) to the nucleotide UAG (which codes for a stop codon).
The translation machinery would reach this codon, which signals that translation should be terminated. This would mean that the mRNA would be released, and a truncated (shortened) version of the amino acid would be produced. This is likely to be non-functional, and may even be marked for destruction by the cell.
Answer:
Which of the following situations would be most likely to engage the anterior cingulate cortex?
A waiter walks over to your table at a restaurant and holds an open menu in front of you.
You walk into the elevator of your apartment building and press the button for your floor.
You enter your classroom and find someone sitting in your usual seat.
A good friend asks you to remind him of your telephone number.
Explanation:
In order to meet the demand of the surrounding environment by adjusting the motor outputs from the brain to the environments ,a part of the emotion control part of the brain( limbic system) called the anterior cingulate cortex is responsible for this.
Anatomically, the limbic system is made up of the primarily the amygdala, hippocampus, thalamus, hypothalamus, basal ganglia and cingulate gyrus.
Based on the this its(anterior cingulate cortex) functions are formation of emotion in the limbic system, and possessing of memory and learning.
it is also a major parts of of pain networks for pain modulation and perception.
Therefore whenever there is change in the environment from the prestored condition in the brain,(e.g the usual seat in the class room occupied above) the anterior cingulate cortex adjust the motor input from the brain to adapt to the new environment to take an action,whih in this case leads to the reclaiming of the seat or any other necessary proactive action.
