Question

(c) Describe the hormone and signaling protein combination that produced the greatest and most prolonged response. For the phosphorylated STAT5 response that reached 100 percent at 10 minutes in figure 1A, calculate the rate of decrease (percent decrease per minute) in the detected level of phosphorylated STAT5 from 10 to 30 minutes.

Answer 1

Answer:

Insulin and protein kinase B interaction is the greatest and most prolonged response as is seen in the graph. Protein kinase B level reached to 100 after 30 minutes as insulin added while the level remained to near 80 even after 240 minutes.

The level of STAT 5 at the 10th minute = 100

The level of STAT 5 at 30th minute = 35

From 10 to 30 minutes, the level of STAT 5 = 100 – 35

From 10 to 30 minutes, the level of STAT 5 = 65

Duration = 30 – 10 minutes

Duration = 20 minutes

Rate of decrease = 65/20

Rate of decrease = 3.25 levels/min