Answer:
a) The response indicates that a pH below or above this range will most likely cause enolase to denature/change its shape and be less efficient or unable to catalyze the reaction.
b)The response indicates that the appropriate negative control is to measure the reaction rate (at the varying substrate concentrations) without any enzyme present.
c)The response indicated that the enolase has a more stable/functional/correct/normal protein structure at the higher temperature of 55°C than at 37°C because the enzyme is from an organism that is adapted to growth at 55°C.
Explanation:
Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis.In bacteria, enolases are highly conserved enzymes and commonly exist as homodimers.
The temperature optimum for enolase catalysis was 80°C, close to the measured thermal stability of the protein which was determined to be 75°C, while the pH optimum for enzyme activity was 6.5. The specific activities of purified enolase determined at 25 and 80°C were 147 and 300 U mg−1 of protein, respectively. Km values for the 2-phosphoglycerate/phosphoenolpyruvate reaction determined at 25 and 80°C were 0.16 and 0.03 mM, respectively. The Km values for Mg2+ binding at these temperatures were 2.5 and 1.9 mM, respectively.
Enolase-1 from Chloroflexus aurantiacus (EnoCa), a thermophilic green non-sulfur bacterium that grows photosynthetically under anaerobic conditions. The biochemical and structural properties of enolase from C. aurantiacus are consistent with this being thermally adapted.
DNA replication has such accuracy varies. There are many DNA polymerases/repair enzymes that synthesize/repair DNA. Some are more frequent than others. The error frequency ranges between 1 to per 10,000 base pairs to 1 to per 10,000,000 base pairs, depends on the enzyme
Answer:
During an investigation, the addition of radiolabeled amino acids during protein synthesis occurs when the data shows high levels of radiation in protein synthesis, packaging and transport, which is equivalent to saying that radioactivity levels would first increase in the rough endoplasmic reticulum, followed by the Golgi and then the secretory vesicles (option C).
Explanation:
Protein synthesis in the cell is a process that involves the formation of polypeptide chains with the successive incorporation of amino acids.
RNA, which contains the sequence of triplets or codons that form the genetic code, is coupled to the ribosomes. Each codon or triplet consists of three nucleotides and encodes specific amino acids.
The RNA chain contains a specific nucleotide sequence and determines the assembly of amino acids to a polypeptide chain, by the action of ribosomes present in the rough endoplasmic reticulum.
In the Golgi apparatus, the proteins formed are conjugated, with the addition of carbohydrates or lipids, and arranged in secretory vesicles for transport.
In these steps, where the labeled amino acids are present, the levels of radioactivity would increase in the rough endoplasmic reticulum, the Golgi apparatus and the secretory vesicles.
Learn more:
Protein synthesis steps brainly.com/question/884041
Your PSYCHROMETER indicates that the relative humidity is very low. Rain probably WON'T occur.
Answer:
9 cm for controlled, 8cm for experimental, and -1 for the last one that pops up when you submit one and two.
Explanation:
I got it right on EDG.2020
