Answer:
According to the result recorded, there was no cholesterol in the albumin solution. This is probably because the cholesterol is in the egg yolk and not the egg white. In order to get a positive response on the presence of cholesterol in eggs, an egg yolk albumin stock needs to be prepared. So using the same procedure as before and only replacing the egg yolk for the egg white, prepare a 0.5 mL of egg yolk with 4.5 mL distilled water mixture in a test tube. Follow the steps given in the lab manual to check for presence of lipids in this albumin stock. Take a filter paper and add one drop of Sudan III solution onto it, let it dry and then place a drop of the stock prepared and analyze if the color transfer happens.
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Answer:
C. DNA ligase adds nucleotides to the lagging strand
Explanation:
DNA replication is the process during cell division in which DNA copies itself. DNA strands unwind with the help of helicase to initiate the process. DNA Polymerase III is responsible for prokaryotic replication and adds nucleotides in 5' to 3' direction. Since both the strands of DNA run in opposite direction their replication is slightly different. The lagging strand is also formed by DNA Polymerase III in discontinuous manner leading to formation of Okazaki fragments. DNA ligase joins these fragments once the replication process is completed.
DDT stands for dichloro-diphenyl-trichloroethane. The first kind of synthetic/artificial insecticides came into use in the 1940s. The earlier usage of DDT include: a) Killing of malarial vectors, b) Combatting Typhus and other insect borne human diseases, c) As a pest control in crops d) as a pest control in garden, live stock production and even at homes.
The negative impact of DDT could be felt for the first time when the pests that were earlier killed by use of DDT have now become pesticides resistant. In the 1950s in USA, the regulatory measures were adopted to reduce the usage of DDTs as its effects as a pesticides were no more long significant and also it was creating detrimental physical and psychological impacts on the human and environment.
It was in 1972 that the U.S. Environmental Protection Agency cancelled the order for banning the usage of DDT based on the adverse impact it produced on the environment, human and other life forms. Since then continuous studies are being conducted to analyse the impact of DDTs. In some later years it was established that DDT is the cause of producing tumors in liver.
Some of the common negative impacts produced by DDT as per the U.S. Department of Agriculture :
a) The non destructive nature – DDT can not be destroyed and thus it remains persistent in the atmosphere
b) It attacks the tissues of living organisms especially the animals and humans ( fatty tissue)
c) It can penetrate the atmosphere to deeper extent.
Now as per the current stuation, The use of DDT is controlled and other alternatives of pest control organisms is being deduced. As per the treaty of Stockholm Convention on POPs (Persistent organic pollutants) , usage of DDT for malarial control is justified but it puts a restrictive use of DDT as pesticides in other areas.
Lysosomes contain about fifty different enzymes that break down all types of biological molecules including proteins, nucleic acids, lipids, and carbohydrates.
Answer:
1. Cartilaginous joints
2. Freely movable (Synovial) joints
3. Temporomandibular joint
4. Arthritis
5. Synchondrosis
Explanation:
1. Cartilaginous joints are those who permits some movements but provide less stability that immovable joint, these joints are slightly movable.
2. Freely movable joints are synovial joints. These joints have a joint cavity between connecting bones, and allow for greater mobility. However, they are less stable than fibrous and cartilaginous joints. For example: elbow.
3. Temporomandibular joint is a like a hinge and a ball and socket joint at the same time. It is a simple hinge that permits to chew.
4. Arthritis is a term that describes inflammation in joints. Some of them include: rheumotaoid arthritis, psoriatic arthritis, etc.
5. Synchondrosis is a joint in which the articulating surfaces are close together but are bound by a hyaline cartilage, which can converts to either bone o fibrocartilage.