The DNA, mRNA and tRNA are the essential nucleic acids that initiate protein synthesis. The DNA contains the genetic code of our human body in the form of chromosomes. It is composed of nucleic acids like uracil (U), guanine (G), adenine (A) and cytosine (C). The messenger RNA or mRNA carries this code called codons expressed in three-letter codes. Each amino acid has its own assignment of code. The mRNA carries this code to the ribosome which is the site for protein synthesis. The translational RNA or tRNA contains anti-codons to translate the codes in the mRNA into amino acids that link together to form proteins.
Answer:
a) The response indicates that a pH below or above this range will most likely cause enolase to denature/change its shape and be less efficient or unable to catalyze the reaction.
b)The response indicates that the appropriate negative control is to measure the reaction rate (at the varying substrate concentrations) without any enzyme present.
c)The response indicated that the enolase has a more stable/functional/correct/normal protein structure at the higher temperature of 55°C than at 37°C because the enzyme is from an organism that is adapted to growth at 55°C.
Explanation:
Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis.In bacteria, enolases are highly conserved enzymes and commonly exist as homodimers.
The temperature optimum for enolase catalysis was 80°C, close to the measured thermal stability of the protein which was determined to be 75°C, while the pH optimum for enzyme activity was 6.5. The specific activities of purified enolase determined at 25 and 80°C were 147 and 300 U mg−1 of protein, respectively. Km values for the 2-phosphoglycerate/phosphoenolpyruvate reaction determined at 25 and 80°C were 0.16 and 0.03 mM, respectively. The Km values for Mg2+ binding at these temperatures were 2.5 and 1.9 mM, respectively.
Enolase-1 from Chloroflexus aurantiacus (EnoCa), a thermophilic green non-sulfur bacterium that grows photosynthetically under anaerobic conditions. The biochemical and structural properties of enolase from C. aurantiacus are consistent with this being thermally adapted.
The flowering plant does not wilt but stands straight up reaching toward the sunlight because its cells contains mostly... Water. The answer is Water. I hope this helps!
Answer:
0.05 mg/mL ( B )
Explanation:
Given data:
20 mg/ml starch
2% solution = 2g of solute is in 100g of solvent
<u>Determine the new concentration in mg/ml </u>
Dilution equation = C1V1 = C2V2
new concentration ; applying the dilution factor
dilution factor = 1 : 400 ; ( 2 /400 )g = 0.005 g of solute is present in every 100 mL
∴ new concentration = 0.00005 g / 1 mL * ( 1000 mg / 1g ) = 0.05 mg/mL
