Answer:
A) Contains DNA
Explanation:
See it this way, which one would make more sence?
Answer:
C. Substance B gives off OH– ions that combine with H+ ions when dissolved in water.
Explanation:
A base is a substance that interacts with water to yield excess hydroxide ions in an aqueous solution.
Bases are certain metallic oxides, metallic hydroxides and aqueous ammonia.
Bases reacts with acids to produce salts which is substance C as described in first option.
Substance A is an acid and it gives off Hydrogen ion.
Full information in question:
CD3 is a signaling protein that is typically found only in the plasma membrane of immune system T lymphocytes. CD3 is composed of several different polypeptides, including a gamma chain, CD3γ . Scientists analyzed the promoter of the CD3γ chain gene for regulatory sequences that might have positive or negative effects on expression of the gene. The scientists cloned fragments of the CD3γ gene that included the first transcribed nucleotides plus up to 789 nucleotides of upstream regulatory sequences into plasmids in which the gene for the firefly enzyme luciferase immediately follows the fragments. The plasmids were then introduced into a line of T lymphocytes (Figure 1), and the cells were allowed to grow for a short while. Because the regulatory sequences of the CD3γ gene immediately precede the luciferase gene in the plasmids, the activity, either positive or negative, of the regulatory sequences affected the amount of luciferase gene expression by the T lymphocytes. Luciferase catalyzes a reaction that results in the release of light and is responsible for the bioluminescence (light flashes) of fireflies. By quantifying the bioluminescence, or luciferase activity, in the cells, the scientists were able to determine the effects of each CD3γ gene fragment cloned into the plasmids (Figure 2) on expression of the gene.
Answer:
Luciferase activity will be absent
Explanation:
The regulatory element introduced into the plasmid that controls the luciferase activity is lymphoid specific. That means it is only active in lymphoid cells, controlling the expression of lymphoid-specific genes. It works because the correct combination of transcription factors and other regulatory proteins are present in lymphoid cells to bind to the promoter and make it active. This means the promoter can express the luciferase gene.
If it was inserted into kidney cells, the promoter would not be active. The correct combination of regulatory proteins would not be present, and would not be able to turn on the promoter. Therefore, luciferase would not be active.
Answer:
There is not enough oxygen in the culture fluid
Explanation:
The presence of oxygen is required by living beings to continue the process of aerobic cellular respiration which in turn generates energy by oxidation of nutrients. Here, oxygen serves as a terminal electron acceptor during electron transport from NADH and FADH2 which are formed during glycolysis and Kreb's cycle. When oxygen is not available to accept the electrons, cells cannot continue the process of aerobic cellular respiration.
Cells enter the alternative pathway of energy production which is the fermentation. During lactic acid fermentation, pyruvate formed by glycolysis accepts an electron from NADH. The result is the formation of lactate and NAD+. This recycling of NAD+ ensures the continuation of glycolysis to produce ATP molecules.
Accumulation of lactic acid in the culture fluid indicates the fact that the cells are not getting enough oxygen to perform aerobic cellular respiration and have entered lactate fermentation.
