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seraphim [82]
2 years ago
13

In the first part of the dna isolation, you discarded the supernatant and kept the cells. however, after processing the sample w

ith chelex, you kept the supernatant and discarded the pellet. tracing the path of your dna, explain the goal of each processing step.
Biology
1 answer:
pishuonlain [190]2 years ago
8 0
<span>For the first step, you must isolate the cells from the media. The cells contain the DNA so you keep the cells and pellet. The next step would be a cell lysis which causes the cells to open and the DNA to come out. At this point you would keep the supernatant, as the pellet is the cell membrane and other parts of the cell. The chelex is to bind to transition metal ions. This would cause and DNases in the cell to become inactive because the metal ion is their cofactor. The pellet in this case would contain the chelex and the DNA would still be in the supernatant.</span>
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Which of the following choices correctly matches a tool and its proper application?
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Answer:

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Explanation:

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2 years ago
many different types of mutations can occur within the body .cystic fibrosis is a genetic disorder that is caused by different m
denpristay [2]
<h3>Question from alternative source</h3>

Many different types of mutations can occur within the body. Cystic fibrosis is a genetic disorder that is caused by different mutations within the CFTR gene. One mutation in the CFTR gene that leads to cystic fibrosis causes translation to end earlier in the gene sequence. Which type of mutation causes this translation error that leads to cystic fibrosis?

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Answer:

Nonsense

Explanation:

Nonsense mutations describe mutations that produce a premature stop codon. The stop codons are UAG, UAA, and UGA. A way in which this could arise is, for example, if there was a substution of a nucleotide in the mRNA codon UAC (which codes for the amino acid Tyrosine) to the nucleotide UAG (which codes for a stop codon).

The translation machinery would reach this codon, which signals that translation should be terminated. This would mean that the mRNA would be released, and a truncated (shortened) version of the amino acid would be produced. This is likely to be non-functional, and may even be marked for destruction by the cell.

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velikii [3]

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Homologous structures can be defined as similarities between organs or skeletal of organisms which suggest that they might have a common ancestor in the past. Homologous structures will have alike structures although they may be present in animals of different texa.

For example, The front legs of a frog, which is an amphibian is similar to alligator which is a reptile. This common structure show that they have homologous structures and hence might have had a common ancestor in the past.  

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6 0
2 years ago
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Cerrena [4.2K]
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