1. Action potential reaches the axon terminal and depolarizes it.
2. Depolarization opens voltage-gated calcium channels, enabling influx of Ca into the neuron.
3. Calcium binds to specialized proteins on vesicles (containing pre-made acetylcholine) and triggers them to fuse with the neuron membrane at the synapse.
4. Exocytosis of acetylcholine into the synaptic cleft occurs.
5. Acetylcholine diffuses across the synapse and binds to nicotinic receptors on the end plate of the myocyte.
6. Activated nicotinic receptors, themselves ion channels, cause cation influx into the myocyte and generate an end plate potential. This eventually gives rise to the full depolarization within the myocyte that enables contraction.
Answer:
the reporter gene can randomly insert near to an enhancer sequence which can induce its expression
Explanation
Enhancers are genetic sequences capable of activating gene expression by binding to specific proteins (e.g., transcription factors). Enhancers can regulate the expression of nearby genes located thousands of nucleotides away, i.e., over several kilobases away. In the human genome, it is well known that enhancers are scattered across the 98% of the genome. In this case, it is expected that the reporter GFP gene construct is randomly inserted near an enhancer sequence (a 10% chance of insertion), thereby being regulated by that enhancer.
This indicator is an estimate of the amount of space on the earth that an individual uses in order to survive using existing technology. This space includes the biologically productive land and water area that produces the resources consumed by that individual such as food, water, energy, clothing, and building materials. It also includes the amount of land and water required to assimilate the wastes generated by that person. In other words, the ecological footprint measures a person's demand on the bio-capacity of the Earth.
I'd say the answer is suffer from emotional abuse or be an overachiever
