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Elina [12.6K]
1 year ago
5

For each set of dilutions (bleach and mouthwash), the first tube contained a 1:11 dilution (0.5 mL of agent was added to 5.0 mL

of nutrient broth). What was the dilution of the MIC for the mouthwash and the bleach? Hint: You will have to calculate the dilution of each tube 2 – 4.
Chemistry
1 answer:
lidiya [134]1 year ago
5 0

Answer:

Tube 2: 8.26 * 10^-3; Tube 4: 6.83 * 10^-5

Explanation:

In the serial dilutions for MIC test, the volume of nutrient broth in each tube should be equal: 5.0 mL. And the volume of agent in each dilution should also be similar: 0.5 mL.

The serial dilutions was as following:

  • Tube 1: 0.5/5.5
  • Tube 2: 0.5 mL of tube 1 was diluted with 5.0 mL broth. Then, the dilution of tube 2 is (1:11) * (1:11) = (0.5/5.5) * (0.5/5.5) = 1:121 = 8.26 * 10^-3
  • Tube 3: We perform the similar calculation. Thus, the result is 1:1331 = 7.51 * 10^-4
  • Tube 4: It is 1:14641 = 6.83 * 10^-5.

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Complete combustion of a compound containing hydrogen and carbon produced 2.641 g of carbon dioxide and 1.442 grams of water as
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  The empirical   formula  of hydrocarbon is C₃H₈

The  molecular formula of hydrocarbon   is C₆H₁₆


<u><em> Empirical  formula  calculation</em></u>

Hydrocarbon  contain  carbon and hydrogen

  Step 1:  find the  mass  carbon (C) in carbon dioxide (CO₂)  and hydrogen (H )  in water

mass of  of element = molar mass  of element/ molar mass molecule x total mass of    molecule

From periodic table the molar mass  of C =12,    for CO₂ = 12+( 16 x2) =44 g/mol,     for H = 1.00 g/mol,    for H₂O = (2 x1)+16 = 18 g/mol

mass of C = 12/44 x 2.641 =0.7203 g

since there are 2 atom  of H in H₂O the molar mass of H = 1 x2 = 2 g/mol

mass of H  is therefore =  2/18 x 1.442 =0.1602 g


Step 2:  find the moles of C and H

moles = mass÷ molar mass

moles of C = 0.7203 g÷ 12 g/mol = 0.060  moles

moles of H  =  0.1602÷ 1 g/mol = 0.1602 moles


Step 3: find the mole ratio  of C and H by dividing  each  mole by smallest mole ( 0.06)

for C = 0.06/0.06 =1

  For H = 0.1602/0.06 =2.67

multiply   by 3  to remove the decimal

For C = 1 x3 =3

For H = 2.67 x3 =8

therefore the empirical formula = C₃H₈


<u><em>The molecular formula calculation</em></u>

[C₃H₈]n  = 88.1 g/mol

[12 x 3)+( 1 x8)]n =88.1 g/mol

44 n = 88.1

divide both side by 44

n=2

therefore [C₃H₈]₂   = C₆H₁₆



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